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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has been observed in many tumors, which results in chromosome instability, and therefore may perhaps lead to tumorigenesis. This protein was identified to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important purpose in promoting mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two added splice variants were noted but specific nucleotide sequence information just isn't but out there.

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Blocking peptides are peptides that bind particularly to the target antibody and block antibody binding. These peptide usually includes the epitope acknowledged through the antibody.

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This guideline outlines even further attainable optimization of mobile and immunoassay parameters to ensure the very best effects are...

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This guide outlines even more achievable optimization of mobile and immunoassay parameters to be sure the best possible success are...

Antibodies certain to the blocking peptide no longer bind to your epitope within the target protein. By comparing the staining in the blocked antibody as opposed to the antibody by yourself, you can see which staining is precise.

Goal Data Cyclin E belongs to the hugely conserved cyclin loved ones, whose members are characterized by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation patterns which lead into the temporal coordination of each mitotic function. Cyclin E sorts a posh with and capabilities for a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates for the G1-S section boundary and is particularly degraded as cells development by way of S stage.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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